Defined Media, Defined Results: Scaling E. coli from Bench to Fermenter

Microbial systems are foundational to plasmid DNA and recombinant protein production, yet scaling from development to production often introduces variability, performance limitations, and regulatory complexity—especially when complex media components are involved. As organizations push for higher yields, improved reproducibility, and animal origin free workflows, chemically defined solutions are becoming essential.

In this webinar, Stanislav Pepeliaev Ph.D. will share firsthand insights from beta-testing at the Jenner Institute, with the Gibco™ Bacto CD Supreme Fermentation Production Medium (FPM) Plus and Bacto CD Supreme Feed (2x), a next-generation chemically defined formulation designed to enhance E. coli culture performance. He will discuss observed performance improvements, robustness, workflow integration, and how defined systems can support consistent plasmid DNA and recombinant protein production.

Building on this foundation, fermentation expert Neelanjan (Neel) Sengupta Ph.D. will present a scale-up perspective—exploring how chemically defined media supports transition from bench-scale experiments to controlled single-use fermenter environments. The session concludes with a live Q&A featuring Stan, Neel, and Mark Wight to address implementation strategies and audience questions.

By attending, participants will:

Understand the performance advantages of chemically defined media in E. coli systems
Evaluate real-world beta-test data supporting plasmid DNA workflows
Learn practical scale-up considerations for transitioning to controlled fermenter systems