Simplifying ADC Analysis: Real-Time DAR and Concentration Measurement Without Dilution

Antibody-drug conjugates (ADCs) present unique analytical challenges, particularly in maintaining consistent drug-to-antibody ratio (DAR), minimizing variability, and reducing reliance on time-intensive off-line methods. These challenges are further compounded by the need to limit handling of highly potent payloads while maintaining process control.

This webinar explores how variable pathlength spectroscopy enables rapid, accurate at-line and in-line measurement of both concentration and DAR throughout ADC development and manufacturing.

Through real-world case studies, you’ll see how replacing traditional UV-Vis workflows with slope-based analysis eliminates dilution steps, reduces error, and accelerates decision-making. We will also demonstrate how integrating real-time in-line data supports improved process understanding, tighter control during TFF, and more efficient scale-up.

Join us to learn how applying Process Analytical Technology (PAT) principles can simplify ADC workflows, reduce risk, and drive more consistent process outcomes.

Key Learning Objectives:
How variable pathlength spectroscopy (VPT) works to measure concentration and DAR without dilution
How to apply at-line and in-line VPT to generate real-time data for improved ADC process understanding
How to reduce analytical variability, operator handling, and process risk compared to traditional UV-Vis methods